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Fluid shear stress does not alter <t>PAF</t> <t>receptor</t> expression. Neutrophils were exposed to fluid shear stress (1.0 dyn/cm 2 ) for 30 min at 23°C. Flow cytometry histograms ( A ) and mean fluorescence intensities ( B ) of PAF receptor expression in sheared and nonsheared neutrophil samples. ( C ) PAF receptor density of neutrophils exposed to static conditions and fluid shear stress (1.0 dyn/cm 2 ) for 30 min at 23°C. NS , not significant. To see this figure in color, go online.
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Fluid shear stress does not alter PAF receptor expression. Neutrophils were exposed to fluid shear stress (1.0 dyn/cm 2 ) for 30 min at 23°C. Flow cytometry histograms ( A ) and mean fluorescence intensities ( B ) of PAF receptor expression in sheared and nonsheared neutrophil samples. ( C ) PAF receptor density of neutrophils exposed to static conditions and fluid shear stress (1.0 dyn/cm 2 ) for 30 min at 23°C. NS , not significant. To see this figure in color, go online.

Journal: Biophysical Journal

Article Title: Fluid Shear Stress Increases Neutrophil Activation via Platelet-Activating Factor

doi: 10.1016/j.bpj.2014.04.001

Figure Lengend Snippet: Fluid shear stress does not alter PAF receptor expression. Neutrophils were exposed to fluid shear stress (1.0 dyn/cm 2 ) for 30 min at 23°C. Flow cytometry histograms ( A ) and mean fluorescence intensities ( B ) of PAF receptor expression in sheared and nonsheared neutrophil samples. ( C ) PAF receptor density of neutrophils exposed to static conditions and fluid shear stress (1.0 dyn/cm 2 ) for 30 min at 23°C. NS , not significant. To see this figure in color, go online.

Article Snippet: Primary goat anti-human PAF-R antibody, which binds to the extracellular N-terminus of the PAF receptor, and FITC-conjugated secondary donkey anti-goat IgG antibody were purchased from Santa Cruz Biotechnology (Santa Cruz, CA).

Techniques: Shear, Expressing, Flow Cytometry, Fluorescence

Comparison of fluid shear stress effects on neutrophil activation via PAF ( A ) and fMLP ( B ). Shear stress exposure in the microvasculature increases neutrophil activation via PAF ( A ), whereas activation is suppressed upon exposure to fMLP ( B ). ( FPR , formyl peptide receptor; FSS , fluid shear stress; PAF , platelet-activating factor; PAFR , platelet-activating factor receptor.). To see this figure in color, go online.

Journal: Biophysical Journal

Article Title: Fluid Shear Stress Increases Neutrophil Activation via Platelet-Activating Factor

doi: 10.1016/j.bpj.2014.04.001

Figure Lengend Snippet: Comparison of fluid shear stress effects on neutrophil activation via PAF ( A ) and fMLP ( B ). Shear stress exposure in the microvasculature increases neutrophil activation via PAF ( A ), whereas activation is suppressed upon exposure to fMLP ( B ). ( FPR , formyl peptide receptor; FSS , fluid shear stress; PAF , platelet-activating factor; PAFR , platelet-activating factor receptor.). To see this figure in color, go online.

Article Snippet: Primary goat anti-human PAF-R antibody, which binds to the extracellular N-terminus of the PAF receptor, and FITC-conjugated secondary donkey anti-goat IgG antibody were purchased from Santa Cruz Biotechnology (Santa Cruz, CA).

Techniques: Comparison, Shear, Activation Assay